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  November 14, 2016:

FINCH has been used to image three distinct sub-resolution proteins in subcellular Golgi apparatus

In an article published on-line in Nature Photonics (http://www.nature.com/nphoton/journal/vaop/ncurrent/full/nphoton.2016.207.html) researchers at CellOptic, Johns Hopkins University and University of Arkansas for Medical Sciences describe a new advance in FINCH imaging that enables, for the first time, super-resolved FINCH imaging of sub-resolution objects with a high (100x) magnification objective lens at about twice the resolution of standard fluorescence microscopy. Using a FINCH microscope outfitted with a newly invented birefringent lens common path interferometer, human cells stained for three different Golgi proteins were imaged, showing the distinct proteins localized next to each other in far greater clarity and resolution than previously possible with a fluorescent microscope. The images were captured and rendered quickly and produced results that compare favorably to SIM (Structured Illumination) microscopy, a leading super-resolution method. It is anticipated because of the rapid speed possible with this new advance in FINCH microscopic imaging that facile live cell super resolution imaging will soon become possible. While the report is a first for high magnification, high numerical aperture objectives, the method can rapidly produce super resolved images with lenses of any magnification and numerical aperture, making super-resolution FINCH microscopy applicable for a wide variety of biological investigations.

CellOptic, Inc. is currently working under a NIH Phase 2 SBIR grant to build a simple low cost turn-key super-resolution FINCH holographic microscope for use in research lab settings. Production is underway on prototypes to be deployed nationwide in the first quarter of 2017.